Composite

Part:BBa_K5477024:Design

Designed by: Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-26)


pRAD27-AhR - receptor module


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1064
    Illegal SpeI site found at 289
    Illegal SpeI site found at 426
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1064
    Illegal NheI site found at 869
    Illegal SpeI site found at 289
    Illegal SpeI site found at 426
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1064
    Illegal BglII site found at 102
    Illegal BglII site found at 1879
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1064
    Illegal SpeI site found at 289
    Illegal SpeI site found at 426
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1064
    Illegal SpeI site found at 289
    Illegal SpeI site found at 426
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pRAD27-AhR module is used for our biosensor system for detecting environmental pollutants like PAHs and PCBs. Under the presence of these compounds, receptor modules including ARNT () and NCOA () will bind to XRE () and initiate the transcription of NanoLuc () in our reporter module (). The composite part was cloned using the method of USER-cloning into YCp-L. The YCp-L plasmid is a centromeric plasmid that carries a LEU2 marker for leucine selection in yeast. Centromeric plasmids like YCp-L are low-copy vectors, typically maintained at one to two copies per cell due to the presence of a CEN sequence.



Source

Homo sapiens and <Saccharomyces cerevisiae</i>

References