Composite
Part:BBa_K5477024:Design
Designed by: Kate Malana Escobar Group: iGEM24_UCopenhagen (2024-09-26)
pRAD27-AhR - receptor module
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1064
Illegal SpeI site found at 289
Illegal SpeI site found at 426 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1064
Illegal NheI site found at 869
Illegal SpeI site found at 289
Illegal SpeI site found at 426 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1064
Illegal BglII site found at 102
Illegal BglII site found at 1879 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1064
Illegal SpeI site found at 289
Illegal SpeI site found at 426 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1064
Illegal SpeI site found at 289
Illegal SpeI site found at 426 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The pRAD27-AhR module is used for our biosensor system for detecting environmental pollutants like PAHs and PCBs. Under the presence of these compounds, receptor modules including ARNT () and NCOA () will bind to XRE () and initiate the transcription of NanoLuc () in our reporter module (). The composite part was cloned using the method of USER-cloning into YCp-L. The YCp-L plasmid is a centromeric plasmid that carries a LEU2 marker for leucine selection in yeast. Centromeric plasmids like YCp-L are low-copy vectors, typically maintained at one to two copies per cell due to the presence of a CEN sequence.
Source
Homo sapiens and <Saccharomyces cerevisiae</i>